Composite

Part:BBa_K4432304:Design

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)


PANTR1 Toehold Switch Trigger 04 Transcriptional Unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + PANTR1 Toehold Switch Trigger 04 (BBa_K4432204) + the strong SBa_000587 Terminator (BBa_K3453000)

Source

DNA synthesis & Assembly

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.